Catalogue 2016 - 2017

NSCI 401 Genetic & Molecular Tech in Neurosci


4 cr.


NSCI 212 and NSCI 267 or Instructor's Approval

Course Description

This practice-based, laboratory course introduces students to a selection of advanced techniques and methods used in contemporary genetic and molecular neuroscience. The common fruit fly, Drosophila melanogaster, will be examined and utilized as our model organism because it offers unparalleled genetic tools, rapid lifespan turn-over, and relatively simple anatomy and physiology. Some of the techniques that students will practice include: immunohistochemistry and immuno-fluorescence (fixation, primary and second antibody conjugation), fluorescence microscopy (excitation, emission, and filter cube physics), inverted microscopy (physiologic microscopy, activation of synapses and calcium indicator dyes), both end-point and quantitative Polymerase Chain Reaction (PCR), “fly pushing” (produce mutant strains), and evaluation of custom cassette inserts to the fruit fly genome (e.g. GAL4/UAS, GAL80 / temperature sensitive control, and geneswitch lines).